Differential between Protein and mRNA Expression of CCR7 and SSTR5 Receptors in Crohn's Disease Patients

Table 2

Results of the DNA sequencing analysis of PBMCs for SSTR5 in 4 control donors and 4 CD patients. mRNA extraction was realized from PBMC of 4 CD patients and 4 control donors. cDNA was obtained after RT-PCR and PCR. Total cDNA was checked for integrity and concentration using the DNA 7500 LabChip kit on the Agilent 2100 bioanalyzer and for purity by reading the absorbance at 260 and 280 nm with ratios A260/A280 always between 1.8 and 2.0.

Donors	Size (bp)	Average size (bp) ± sd
Control 1	1284	1298±10
Control 2	1310
Control 3	1294
Control 4	1302
CD patient 1	1259	1263±4
CD patient 2	1259
CD patient 3	1266
CD patient 4	1266