A Splice Variant of ASC Regulates IL-1 Release and Aggregates Differently from Intact ASC
Figure 1
Analysis of protein X by mass spectrometry. (a) HL-60 cells were subjected to SDS-PAGE and western blot analysis using antihuman ASC antibody. In addition to full length ASC (fASC), an additional polypeptide of 20 kDa (Protein X) was detected. (b) Proteins that were affinity-purified using anti-ASC antibody were stained with Coomassie Blue. HC: Ig heavy chain, LC: Ig light chain. (c) Mass spectrum of digested protein X is shown. (d) Structures of ASC and Protein X. Homologous regions are underlined. aa: amino acid. (e) Amino acid sequence of fASC from a database (GenBank Accession no. NP 037390) and Protein X is displayed. Homologous sequences are underlined. The amino acid sequence written in light characters was not detected in Protein X. (f) Sequence of ASC cDNA extracted from HL-60 cells as Protein X is shown.
