IL-1β Suppresses the Formation of Osteoclasts by Increasing OPG Production via an Autocrine Mechanism Involving Celecoxib-Related Prostaglandins in Chondrocytes
Figure 3
Effect of IL-1β on OPG gene and protein expression. (a) Cells were cultured with 0, 10, or 100 U/mL IL-1β and OPG gene expression was determined by real-time PCR on days 1, 3, 5, 7, 10, 14, 21, and 28 of culture. (b) OPG protein expression determined by ELISA on days 21 and 28. Each bar indicates the mean SD from four separate experiments. for IL-1β treatment versus the control.
