IL-1β Suppresses the Formation of Osteoclasts by Increasing OPG Production via an Autocrine Mechanism Involving Celecoxib-Related Prostaglandins in Chondrocytes
Figure 6
Effect of IL-1β on osteoclast differentiation estimated by the number of TRAP-positive multinucleate cells. Cells were cultured in DMEM containing 10% FBS with or without 100 U/mL IL-1β for 7 or 28 days. RAW264.7 cells were cultured under the conditions described in Figure 5. TRAP-positive multinucleate cells with more than three nuclei were considered osteoclasts, and the data were converted to the percentage of TRAP-positive multinucleate cells of the total number of cells. Each bar indicates the mean ± SD from separate experiment. for IL-1b treatment versus the control.
