TOM1L Is Involved in a Novel Signaling Pathway Important for the IL-2 Production in Jurkat T Cells Stimulated by CD3/CD28 CoLigation

Figure 8

Jurkat cells were transfected with a firefly luciferase reporter for STAT3 (Signal-transducer-andactivator-of-transcription-3) together with plasmids encoding TOM1L or the empty plasmid construct. Included in each transfection was a reporter construct that expresses Renilla luciferase under the control of the 𝛽-actin promoter. 48 hours after the transfection, the cells were washed and lysed followed by reading in a luminometer. The activity of Renilla luciferase in each sample was used to normalize the luciferase data.