Substance P Increases Cell-Surface Expression of CD74 (Receptor for Macrophage Migration Inhibitory Factor): In Vivo Biotinylation of Urothelial Cell-Surface Proteins
Figure 2
Epithelial
aggregate separation and isolation (EASI) of rat bladder urothelium. Hematoxylin
staining of scraped bladders. (a) and (b) saline-treated bladders, (c) and (d)
SP-treated bladders. EASI only removes urothelium leaving intact lamina
propria. Calibration bar—(a), (c) =
400 μm; (b), (d) = 100 μm. (e) Vimentin Western blot of total urothelial cell
lysates. Total protein from isolated urothelial cells was separated on 4–12% bis tris
acrylamide gels and transferred to
PVDF. Lanes 1–6 saline-treated
animals; lanes 7–12 SP-treated
animals, lane S—10 μg of scrapped
bladder protein (positive control). Vimentin Western blot was stripped and
reprobed with GAPDH which served as a loading control.
