Substance P Increases Cell-Surface Expression of CD74 (Receptor for Macrophage Migration Inhibitory Factor): In Vivo Biotinylation of Urothelial Cell-Surface Proteins
Figure 4
(a) MIF and CD74
mRNA expressions in
isolated urothelial cells. Total RNA was isolated from urothelial cells and CD74
or MIF-specific primers used to amplify mRNA by endpoint PCR. A CD74 and MIF
mRNA is increased in isolated urothelial cells after SP treatment. Data are
representative of 5 salines and 5 SP-treated animals. 18S rRNA was used as a
control for the amount of total RNA added to each reaction tube. (b) Relative
expression of CD74 and MIF in isolated urothelial cells. Data are expressed as
fold change calculated by determining mean gene expression ratio (net intensity
of gene-specific band divided by net intensity of 18S rRNA band) normalized to
saline controls. (, n = 5).
