Substance P Increases Cell-Surface Expression of CD74 (Receptor for Macrophage Migration Inhibitory Factor): In Vivo Biotinylation of Urothelial Cell-Surface Proteins
Figure 5
CD74 confocal
microscopy. Frozen
bladder sections (14 μm; intact bladders) were exposed to CD74 antiserum and
visualized using secondary antiserum labeled with FITC or TRITC. Control
experiments included omission of the primary antiserum (panel D). (a) Saline-treated
bladder with 40 objective. (b) SP-treated bladder with 40 objective. (c)
SP-treated bladder with 100 objective. (d)
Saline-treated bladder with 40 objective, secondary antibody only. Excitation wavelength of 488 nm
wasused and emission wavelength of 505 nm and up was collected.
Frame size of the image for (a), (b), and (d) is 230.3 μm 230.3 μm;
optical thickness of the images is 2 μm. Frame size of the image for (c) is
92.1 μm 92.1 μm; optical thickness of the image is 0.9 μm. LP: lamina propria; NUC: nucleus;
arrows indicate luminal surface of urothelial cells.
