Mediators of Inflammation

Research Article

High Levels of Proinflammatory Cytokines, but Not Markers of Tissue Injury, in Unaffected Intestinal Areas from Patients with IBD

Table 2

Real-time PCR information. Primer sequences, PCR products length, temperature of annealing, and source of the primers used for Quantitative PCR.


Gene	Forward primer ()	Reverse primer ()	PCR product length (base pairs)	Annealing temperature	Primer source

-actin	atg ggt cag aag gat tcc tat gtg	ctt cat gag gta gtc agt cag gtc	359	60	M. Bongers, E. Liehl, J. Barsig, Focus 21, 66 (2006)
GZMB	aag acg act tcg tgc tga ca	ccc caa ggt gac att tat gg	62	60	Universal Probe Library Human#60 (Roche)
IFN	tgg aaa gag gag agt gac ag	att cat gtc ttc ctt gat gg	129	60	M. G. Karlsson, J. Ludvigsson, Diabetes Res. Clin. Pract. 40, 21 (1998)
IL-1	tcc gac cac cac tac ag	cgg agc gtg cag ttc a	224	53	Designed with the software LightCycler Probe Design 2 (Roche)
IL-4	ttc tac agc cac cat gag	cat gat cgt ctt tag cct ttc	198	59	Designed with the software LightCycler Probe Design 2 (Roche)
IL-6	Commercial reagent		—	60	TaqMan Gene Expression Assay Hs00174131_ml (Applied Biosystems)
IL-10	Commercial reagent		—	60	TaqMan Gene Expression Assay Hs00174086_ml (Applied Biosystems)
IL-12(p35)	tgt cac cga gaa gct gat gt	gag gtt tct ggc caa act ga	278	68	Designed with the software LightCycler Probe Design 2 (Roche)
IL-12(p40)	Commercial reagent		—	60	TaqMan Gene Expression Assay Hs00233688_ml (Applied Biosystems)
IL-15	tgt ctt cat ttt ggg ctg ttt ca	tcc tcc agt tcc tca cat tct ttg	327	60	C. Kebelmann-Betzing et al., Cytokine 13, 39 (2001)
IL-18	gcttga atc taa att atc agt c	caa att gca tct tat tat cat g	335	55	T. Tomita et al., J. Infect. Dis. 183, 620 (2001)
IL-23(p19)	agc agc tca agg atg gca ctc ag	ccc caa att tcc ctt ccc atc ta	251	55	A. Wesa et al, BMC Immunol. 3, 14 (2002)
IL-27(p28)	gcg gaa tct cac ctg cca	gga aac atc agg gag ctg ctc	69	64	S. Pflanz et al, Immunity 16, 779 (2002)
iNOS	tct gca gac acg tgc gtt act	atg cac agc tga gca ttc ca	115	56	M. Ohtsuki et al., Clin. Chim. Acta 353, 103 (2005)
MMP-3	gaa atg cag aag ttc ctt gg	gtg aaa gag acc cag gga gtg	489	60	T. Sadowski, J. Steinmeyer, Inflamm. Res. 50, 175 (2001)
SOCS-2	agt gtg gtt cat ctg atc g	aca ttt gtt aat ggt gag cct	162	54	Designed with the software LightCycler Probe Design 2 (Roche)
SOCS-3	ggc cac tct tca gca tct c	atc gta ctg gtc cag gaa ctc	109	62	N. Torpey et al, J. Biol. Chem. 279, 26789 (2004)
SOCS-4	cct atg act ggc tct gt	gct tcg gct gcg tat t	269	52	Designed with the software LightCycler Probe Design 2 (Roche)
SOCS-5	cct aca ggt gtt cag taa gac	cca cac tgt tga aat act cat cc	171	56	Designed with the software LightCycler Probe Design 2 (Roche)
TGF₁	gga cac caa cta ttg ctt cag	tcc agg ctc caa atg tag g	148	60	W. X. Chen et al., World J. Gastroenterol. 8, 379 (2002)
TIMP-1	Commercial reagent		—	60	TaqMan Gene Expression Assay Hs00171558_ml (Applied Biosystems)
TNF	tca gat cat ctt ctc gaa cc	cag ata gat ggg ctc ata cc	361	60	G. J. Atkins et al., Bone 26, 653 (2000)