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Mediators of Inflammation
Volume 2009 (2009), Article ID 689430, 8 pages
http://dx.doi.org/10.1155/2009/689430
Research Article

Analysis of Several mRNA in Human Meningiomas

1UMR CNRS 6101, Centre National de la Recherche Scientifique, Faculté de Médecine, Université de Limoges, 2 rue Dr. Marcland, 87025 Limoges, France
2Service d'Anatomie Pathologique, CHU Dupuytren, 87045 Limoges, France
3Service de Neurochirurgie, CHU Dupuytren, 87045 Limoges, France

Received 7 September 2009; Revised 25 November 2009; Accepted 21 December 2009

Academic Editor: Muzamil Ahmad

Copyright © 2009 Yves Denizot et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In view of the important oncogenic action of phospholipase we investigated transcripts in human meningiomas. Real-time PCR was used to investigate transcripts in 26 human meningioma tumors. Results indicated that three -dependent high molecular weight ( -IVA, -IVB, -IVC), one -independent high molecular weight ( -VI) and five low molecular weight secreted forms of ( -IB, -IIA, -III, -V, and -XII) are expressed with -IVA, -IVB, -VI, and -XIIA as the major expressed forms. -IIE, -IIF, -IVD, and -XIIB are not detected. Plasma ( -VIIA) and intracellular ( -VIIB) platelet-activating factor acetylhydrolase transcripts are expressed in human meningiomas. However no difference was found for transcript amounts in relation to the tumor grade, the subtype of meningiomas, the presence of inflammatory infiltrated cells, of an associated edema, mitosis, brain invasion, vascularisation or necrosis. In conclusion numerous genes encoding multiples forms of are expressed in meningiomas where they might act on the phospholipid remodeling and on the local eicosanoid and/or cytokine networks.