The Discovery of Novel Experimental Therapies for Inflammatory Arthritis
Figure 4
The miR Pathway. Step of the miR pathway involves the transcription of a 70–100 nucleotide (nt) pri-miR from an miR gene which is then processed in the nucleus via Step by the RNAse III enzyme, DROSHA to yield pre-miR. Following the transport of pre-miR from the nucleus to the cytoplasm mediated by the nuclear export receptor protein, EXPORTIN 5 at Step , a second RNAse III enzyme, DICER, digests pre-miR at Step resulting in a 21–25 nt miR. At this juncture, miR has the capacity to bind to the RNA-Induced Silencing Complex (RISC) and at Steps and aligns itself with an mRNA. The final step (Step ) is dependent on whatever complimentarity exists between a miR and a target sequence in mRNA. Therefore, the degree of complimentarity between a miR and an mRNA results in either translational repression or mRNA cleavage (reviewed in [109]).
