Leptin Induces an Inflammatory Phenotype in Lean Wistar Rats
Figure 2
The relative mRNA expression of MCP-1 and P-selectin. Expressions of (a) MCP-1 and (b) P-selectin were assessed using pre-developed assays for real-time PCR according to the manufacturer’s instructions (Applied Biosystems). Values were calculated using a comparative method and presented as mean ± SEM of at least 4 observations/group. Statistical analysis was performed using student’s test. P < .05 saline controls.