Myeloperoxidase Content is a Marker of Systemic Inflammation in a Chronic Condition: The Example Given by the Periodontal Disease in Rats
Leukocyte recruitment and myeloperoxidase activity in gingivomucosal tissue surrounding rat molar tooth from naive, nontreated and periodontal-diseased treated rats. Either celecoxib (12 mg/kg/day) or morphine (1 mg/kg/day) was administered subcutaneously (0.1 mL per 100 g body weight) once a day, for 3 consecutive days, from 3rd to 5th day after ligation. Animals were killed on the 11th day of ligation. Sterile physiological saline was given in the same volume and route of administration in control animals. Naive (N) animals were left nonligated. Gingivomucosal tissue samples of ligated (ipsilateral) and non-ligated (contralateral) second molar tooth ( m) were removed from naive, and drug-treated rats and were prepared for histological analysis (Panels (a) and (b)) and MPO activity (Panels (c) and (d)) as described in Materials and Methods. In panels (a) and (b) indicates a significant difference (.05) from contralateral side and in panels (c) and (d) and # indicate significant difference (.05) in relation to naive and control group, respectively; using one-way ANOVA followed by Student-Newman-Keuls test; 4–6 animals per group.
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