Figure 5: Quantitative evaluation of the IL-8, growth related oncogene-α (GROα), and vascular endothelial growth factor (VEGF) released by HT-29 and FaDu in 48 hours. Culture medium (DMEM plus 10% FBS) was replaced and collected after 48 hours. Protein levels were determined by ELISA. Bars represent the mean SEM, . Statistical significance was assessed using Students t-test; when compared with HT-29.