Linking Innate and Adaptive Immunity: Human V9V2 T Cells Enhance CD40 Expression and HMGB-1 Secretion
Figure 3
Modulation of CD25 and CD28 on T cells by IPP-activated T cells. PBMCs were cultured overnight in the presence of IPP or absence of IPP before stimulation with 1 nM of TSST-1 (a). Data (mean SEM) for expression of CD25 and CD28 were shown after various time intervals following TSST-1 stimulation, and the numbers of healthy donors studied for each coreceptor were as indicated. Baseline values were obtained from PBMC treated with RPMI growth medium alone. To determine the influence of IPP-activated T cells on receptor expression, comparisons were made between IPP + TSST-1 (dashed red lines) versus TSST-1 alone (solid black line) and significant differences were denoted by (). To confirm that any observed difference in receptor expression was due to the presence of activated T cells, a cognate group of PBMCs was depleted of T cells (<0.1%) and studied in parallel experiments (b). To determine the influence of TSST-1 on IPP-activated T cells, comparisons were made between IPP + TSST-1 versus IPP alone, and significant differences were denoted by (). There was no additional effect of IPP-activated T cells on CD25 and CD28 expression following TSST-1 stimulation; however, CD25 expression on T cells following TSST-1 stimulation in the presence of IPP-activated T cell was significantly higher at 24 hour than with IPP alone (, student’s -test).
