Cathepsin G, a Neutrophil Protease, Induces Compact Cell-Cell Adhesion in MCF-7 Human Breast Cancer Cells
Figure 7
Guanylyl cyclase inhibitor LY83583 has an inhibitory effect on cathepsin G-promoted E-cadherin-mediated cell-cell adhesion. MCF-7 cells were incubated in 5% FBS-containing medium on fibronectin for 24 hours. Cathepsin G-induced cell condensation was analyzed at the original magnification: 200. (a) After washing, adherent cells were treated with cathepsin G in the absence or presence of 1 M LY83583 for 24 hours. Cells were then analyzed by phase-contrast microscopy at 3 hours and 24 hours. (b) After washing, adherent cells were treated with cathepsin G in the absence or presence of LY83583 for 3 hours. The immunocomplexes were then analyzed by immunoblotting using an anti--catenin antibody. (c) Adherent cells were treated with 1 M 8-Br-cGMP, and cells were then analyzed by phase-contrast microscopy at 3 hours and 24 hours. (d) After washing, adherent cells were treated with cathepsin G in the absence or presence of 1 M PKGI for 24 hours. Cells were then analyzed by phase-contrast microscopy at 3 hours and 24 hours.