The Effects of Interleukin-1β in Tumor Necrosis Factor-α-Induced Acute Pulmonary Inflammation in Mice
Figure 1
Neutrophil accumulation in mouse lungs exposed to TNF-α. Wildtype (WT) or IL-1 knockout (KO) mice were instilled into the oropharynx (OP) with saline (50 L) or rmTNF-α (5 g in 50 L saline/mouse). After 6 hours, lungs were formalin fixed and neutrophil antigen-positive cells were identified by immunohistochemistry (arrows). Images were captured through 10x objective. In the graphical analysis, a neutrophil index was determined as the ratio of neutrophil-positive cells per total number of nuclei per low power field. Three to twelve random fields were analyzed per lung section. Data are represented as mean ± SEM, n = 9–11 lung sections per TNF group. represents versus saline controls or (brackets) between cytokine-treated mice.
