Mediators of Inflammation / 2009 / Article / Fig 1

Research Article

The Effects of Interleukin-1β in Tumor Necrosis Factor-α-Induced Acute Pulmonary Inflammation in Mice

Figure 1

Neutrophil accumulation in mouse lungs exposed to TNF-α. Wildtype (WT) or IL-1 knockout ( KO) mice were instilled into the oropharynx (OP) with saline (50  L) or rmTNF-α (5  g in 50  L saline/mouse). After 6 hours, lungs were formalin fixed and neutrophil antigen-positive cells were identified by immunohistochemistry (arrows). Images were captured through 10x objective. In the graphical analysis, a neutrophil index was determined as the ratio of neutrophil-positive cells per total number of nuclei per low power field. Three to twelve random fields were analyzed per lung section. Data are represented as mean ± SEM, n = 9–11 lung sections per TNF group. represents versus saline controls or (brackets) between cytokine-treated mice.
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(a) WT Sal
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(b) WT TNF-
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(c) IL-1 KO Sal
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(d) IL-1 KO TNF-
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(e)

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