Mediators of Inflammation / 2009 / Article / Fig 2

Research Article

The Effects of Interleukin-1β in Tumor Necrosis Factor-α-Induced Acute Pulmonary Inflammation in Mice

Figure 2

Detection of soluble receptors in WT and IL-1 KO mice. WT or IL-1β KO mice were OP instilled with either saline (50  L) or TNF-α (5  g/50  L/mouse). After 6 hours, serum, BALF, and lung homogenate were isolated and analyzed for ((a), (c), and (e)) TNFR1 or ((b), (d), and (f)) TNFR2 by ELISA, and for lavage and lung, normalized to protein in samples measured by BCA. Results are mean ± SEM, (n = 8–15 mice per group). * represents saline versus TNF-α-treatment of single strain or (brackets) for saline or cytokine-treated comparisons between strains.

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