Rhizoma coptidis Inhibits LPS-Induced MCP-1/CCL2 Production in Murine Macrophages via an AP-1 and NF𝜅B-Dependent Pathway

Figure 2

Binding activity of the transcripton factor AP-1 in nuclear extracts of LPS-stimulated RAW 264.7 cells. Effects of total extract of Rhizoma coptidis was evaluated with different concentrations (incubation time 240 min), (a) and exposure times (dilution 1 : 5), (b) and was compared with transcription factor activation in LPS-stimulated control cells. Values represent results from at least three independent experiments. AP-1: activated protein-1, Co: LPS-stimulated control cells, and CC: cold competition. ${}^{*}P<.005\text{,}$${}^{**}P<.001$.

(a)

(b)