Disruption of Nrf2 Enhances the Upregulation of Nuclear Factor-kappaB Activity, Tumor Necrosis Factor-𝛼, and Matrix Metalloproteinase-9 after Spinal Cord Injury in Mice

Figure 3

NF-$\kappa$B activity in the spinal cord segments of sham and injured Nrf2 (+/+) and Nrf2 (−/−) mice. (a) Representative X-ray film shows NF-$\kappa$B DNA binding activity by EMSA at 24 h after SCI in sham and injured Nrf2 (+/+) and Nrf2 (−/−) mice. (b) Quantification of NF-$\kappa$B DNA-binding activity was performed by densitometric analysis. The figure indicates that spinal cord NF-$\kappa$B activity was significantly increased after SCI and was greater in Nrf2 (−/−) mice than in Nrf2 (+/+) mice. Data represents mean $\pm$ S.E. ($n=6$ per group). *$P<.01$ versus sham control of the same genotype.  $P<.01$ versus injured wild-type mice.

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