Mediators of Inflammation / 2010 / Article / Fig 8

Research Article

Ghrelin Protection against Lipopolysaccharide-Induced Gastric Mucosal Cell Apoptosis Involves Constitutive Nitric Oxide Synthase-Mediated Caspase-3 S-Nitrosylation

Figure 8

Effect of cNOS inhibitor, L-NAME (LN), on ghrelin- (Gh-) induced caspase-3 S-nitrosylation in gastric mucosal cells exposed to H. pylori LPS. The cells were treated with Gh (0.5 μg/mL) or LN (300 μM) + Gh and incubated for 16 h in the presence of 100 ng/mL LPS. A portion of the cell lysates was processed by biotin-switch procedure for protein S-nitrosylation and, along with the reminder of the lysates, subjected to SDS-PAGE, transferred to nitrocellulose, and probed with anti-caspase-3 antibody. The immunoblots shown are representative of three experiments.
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