Suppression of Inflammatory Mediators by Cruciferous Vegetable-Derived Indole-3-Carbinol and Phenylethyl Isothiocyanate in Lipopolysaccharide-Activated Macrophages

Figure 4

Effects of I3C (a) and PEITC (b) on lipopolysaccharide- (LPS-) activated cytokines released into the medium by RAW 264.7 cells. Cells were cotreated with LPS (330 ng/mL) and various concentrations of I3C and PEITC for 24 hours. The levels of tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and interleukin-10 (IL-10) in the medium were then determined using commercial ELISA kits. Values represent the mean $\pm$ SD from three measurements. Data in the same cytokine group with different letters significantly differ (P $<\hspace{0.17em}\hspace{0.17em}$.05).

(a)

(b)