Possible Implication of Local Immune Response in Darier's Disease: An Immunohistochemical Characterization of Lesional Inflammatory Infiltrate

Table 1

Quantitative data results.

(a) Immunohistochemically-characterized inflammatory infiltrate in Darier’s disease (DD), pemphigus vulgaris (PV), and lichen ruber planus (LRP). Mean percentages (%) +SD and range of values in brackets are reported. $P$ significance is reported at the bottom.

	DD	PV	LRP
	% $\pm$SD (range)	% $\pm$SD (range)	% $\pm$SD (range)
CD20+	$11.1\pm 4.4\mathrm{\hspace{0.17em}\hspace{0.17em}}(1\text{–}20)$	$12.6\pm 3\mathrm{\hspace{0.17em}\hspace{0.17em}}(8\text{–}20)$	$13.2\pm 3.8\mathrm{\hspace{0.17em}\hspace{0.17em}}(5\text{–}25)$
CD3	$90\mathrm{\hspace{0.17em}\hspace{0.17em}}(80\text{–}95)$	$90\mathrm{\hspace{0.17em}\hspace{0.17em}}(85\text{–}95)$	$85\mathrm{\hspace{0.17em}\hspace{0.17em}}(80\text{–}95)$
CD4+	$70\pm 6.2\mathrm{\hspace{0.17em}\hspace{0.17em}}(50\text{–}80)$	$47.8\pm 8.6\mathrm{\hspace{0.17em}\hspace{0.17em}}(30\text{–}60)$	$49.7\pm 5.8\mathrm{\hspace{0.17em}\hspace{0.17em}}(35\text{–}60)$
CD8+	$20\pm 5.4\mathrm{\hspace{0.17em}\hspace{0.17em}}(10\text{–}30)$	$40.6\pm 8.9\mathrm{\hspace{0.17em}\hspace{0.17em}}(25\text{–}55)$	$40\pm 5.7\mathrm{\hspace{0.17em}\hspace{0.17em}}(30\text{–}50)$
Granzyme B+	$3.3\pm 2.7\mathrm{\hspace{0.17em}\hspace{0.17em}}(0.4\text{–}0.8)$	$16\pm 4.8\mathrm{\hspace{0.17em}\hspace{0.17em}}(10\text{–}30)$	$18.3\pm 4.7\mathrm{\hspace{0.17em}\hspace{0.17em}}(10\text{–}30)$
CD25+FOXP3+ Tregs	$26\pm 8.5\mathrm{\hspace{0.17em}\hspace{0.17em}}(8\text{–}40)$	$11.5\pm 4.7\mathrm{\hspace{0.17em}\hspace{0.17em}}(1\text{–}20)$	$12.7\pm 4.7\mathrm{\hspace{0.17em}\hspace{0.17em}}(8\text{–}35)$
CD68+CD123+ pDC	$0.6\pm 0.3\mathrm{\hspace{0.17em}\hspace{0.17em}}(0.2\text{–}1)$	$3.1\pm 1.1\mathrm{\hspace{0.17em}\hspace{0.17em}}(0.9\text{–}5)$	$9.1\pm 1.7\mathrm{\hspace{0.17em}\hspace{0.17em}}(6\text{–}13)$

(b) Mean number (N) $\pm$ SD and range of values in brackets of epidermal (e) and dermal (d) CD1a+ LCs/1 HPF, in DD, PV, LRP, and in the normal skin (NSk). $P$ significance is reported at the bottom.

	DD	PV	LRP	NSk
	$\text{N}\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}\text{SD}\mathrm{\hspace{0.17em}\hspace{0.17em}}(\text{range})$	$\text{N}\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}\text{SD}\mathrm{\hspace{0.17em}\hspace{0.17em}}(\text{range})$	$\text{N}\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}\text{SD}\mathrm{\hspace{0.17em}\hspace{0.17em}}(\text{range})$	$\text{N}\mathrm{\hspace{0.17em}\hspace{0.17em}}\pm \mathrm{\hspace{0.17em}\hspace{0.17em}}\text{SD}\mathrm{\hspace{0.17em}\hspace{0.17em}}(\text{range})$
eCD1a+ LCs	$2.4\pm 2\mathrm{\hspace{0.17em}\hspace{0.17em}}(0\text{–}7)$	$5.4\pm 1.7\mathrm{\hspace{0.17em}\hspace{0.17em}}(3\text{–}10)$	$20.9\pm 3\mathrm{\hspace{0.17em}\hspace{0.17em}}(15\text{–}28)$	$9.9\pm 2.1\mathrm{\hspace{0.17em}\hspace{0.17em}}(7\text{–}14)$
dCD1a+ LCs	$3.4\pm 1.1\mathrm{\hspace{0.17em}\hspace{0.17em}}(2\text{–}7)$	$4.9\pm 1.6\mathrm{\hspace{0.17em}\hspace{0.17em}}(2\text{–}7)$	$18.5\pm 5.5\mathrm{\hspace{0.17em}\hspace{0.17em}}(10\text{–}26)$	$5.7\pm 1.4\mathrm{\hspace{0.17em}\hspace{0.17em}}(4\text{–}8)$

$P$ not significant for: CD3 and CD20, among DD, PV, and LRP groups; and for CD4+, CD25+FOXP3+ Tregs, and CD8+ in PV versus LRP.
For all the other comparisons, $P<.05$.
LCs: Langerhans cells, Tregs: regulatory T cells, and pDCs: plasmacytoid dendritic cells.