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Mediators of Inflammation
Volume 2010 (2010), Article ID 364290, 6 pages
http://dx.doi.org/10.1155/2010/364290
Research Article

Changes in Glucose and Glutamine Lymphocyte Metabolisms Induced by Type I Interferon α

1Department of Physical Education, Federal University of Maranhão, 14040-904 São Paulo, SP, Brazil
2School of Physical Education and Sport, University of São Paulo, 5508-900 São Paulo, SP, Brazil
3Department of Physical Education, Presbyterian University Mackenzie, 01302-907 São Paulo, SP, Brazil
4Institute of Biomedical Sciences, University of São Paulo, 5508-900 São Paulo, SP, Brazil
5Department of Biophysics, Federal University of São Paulo, 04023-062 São Paulo, SP, Brazil
6Department of Science and Technology, Federal University of São Paulo, 12231-280 São José dos Campos, SP, Brazil
7Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, Avenida Arlindo Bettio, 1000, 03828-000 São Paulo, SP, Brazil

Received 19 October 2010; Accepted 8 December 2010

Academic Editor: Giamila Fantuzzi

Copyright © 2010 Francisco Navarro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In lymphocytes (LY), the well-documented antiproliferative effects of IFN-α are associated with inhibition of protein synthesis, decreased amino acid incorporation, and cell cycle arrest. However, the effects of this cytokine on the metabolism of glucose and glutamine in these cells have not been well investigated. Thus, mesenteric and spleen LY of male Wistar rats were cultured in the presence or absence of IFN-α, and the changes on glucose and glutamine metabolisms were investigated. The reduced proliferation of mesenteric LY was accompanied by a reduction in glucose total consumption (35%), aerobic glucose metabolism (55%), maximal activity of glucose-6-phosphate dehydrogenase (49%), citrate synthase activity (34%), total glutamine consumption (30%), aerobic glutamine consumption (20.3%) and glutaminase activity (56%). In LY isolated from spleen, IFNα also reduced the proliferation and impaired metabolism. These data demonstrate that in LY, the antiproliferative effects of IFNα are associated with a reduction in glucose and glutamine metabolisms.