Inhibition of Toll-Like Receptor 2-Mediated Interleukin-8 Production in Cystic Fibrosis Airway Epithelial Cells via the 𝛼7-Nicotinic Acetylcholine Receptor

Figure 2

Nicotine inhibits PTG-induced IL-8 protein expression at concentrations of 10 and 50  $\mu$ M. CFTE29o- cells ($1\times {10}^5$/mL) were stimulated with increasing doses of nicotine (0–50 $\mu$M) for 1 hour. These samples were left untreated (control) or treated with PTG (5 $\mu$g/mL, 24 hours) as indicated. Levels of IL-8 in supernatants were measured by ELISA. Assays were performed in triplicate (* and ${}^{\#}P\le .05$, * versus control, $\#$ versus PTG) $(n=6)$.