Inhibition of Toll-Like Receptor 2-Mediated Interleukin-8 Production in Cystic Fibrosis Airway Epithelial Cells via the 𝛼7-Nicotinic Acetylcholine Receptor

Figure 6

CFTE29o- cells express the α7-nAChR. CFTE29o- cells ($1\times {10}^5$/mL) were grown in chamber slides, Fc-blocked, and labelled with FITC-conjugated anti-α7-nAChR or isotype control antibodies. Cells were counterstained with PI, and α7-nAChR surface expression was quantified by laser-scanning microscopy. (a) Representative histogram of FITC fluorescence comparing isotype (clear) and anti-α7-nAChR (solid) antibody-labelled samples. (b) Histogram showing median channel fluorescence $\pm$ SEM $({}^{*}P\le .05,\hspace{0.17em}n=6)$.

(a)

(b)