Release of Danger Signals during Ischemic Storage of the Liver: A Potential Marker of Organ Damage?
Figure 3
Immunohistochemical analysis of HMGB1 expression in rat livers following cold/warm ischemia. Staining pattern changed upon ischemia and mechanical stress (a). (a1) cold ischemia (CI), (a2) mechanical stress plus cold ischemia (M+CI), (a3) warm ischemia (WI), (a4) mechanical stress plus warm ischemia (M+WI). Relative frequency of hepatocytes with nuclear and cytoplasmic staining (b). In normal liver, only single hepatocytes showed cytoplasmic staining (<1%). The relative frequency increased with ischemia time and reached a peak after 2 h/8 h (85%/95%) of warm/cold ischemia and decreased continuously thereafter. Mechanical stress inflicted on the liver during explantation increased HMGB1 translocation ( versus without mechanical stress groups). Data shown are representative of all tissue samples analyzed at a magnification of 200x. .
