Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages

Table 2

Percentage of ${TLR4}_{}^{+}\text{,}$${IL\text{-}6Ra}_{}^{+}$, and ${Gp130}_{}^{+}$ in ${F4/80}_{}^{+}$ splenocytes.

	F4/80^+ cells (%)
	WT		IL-6 KO
	Young	Aged	Young	Aged
TLR4^+	$23.0\pm 2.8$	$24.7\pm 1.7$	$24.6\pm 2.5$	$23.6\pm 0.4$
IL-6Ra^+	$61.4\pm 1.3$	$60.1\pm 1.5$	$59.1\pm 1.1$	$60.2\pm 2.2$
gp130^+	$35.6\pm 6.3$	$27.2\pm 2.0$	$29.9\pm 4.1$	$31.2\pm 2.4$

Splenocytes were incubated with anti-F4/80 and anti-TLR4 antibodies or with anti-F4/80, anti IL-6Ra, and anti-Gp130 antibodies and analyzed by flow cytometry. Data are shown as mean ± SEM of 4-5 mice per group.