Release of Inflammatory Mediators by Human Adipose Tissue Is Enhanced in Obesity and Primarily by the Nonfat Cells: A Review
Figure 2
Comparison of mRNA expression in isolated omental fat cells versus in vitro differentiated adipocytes. The data are shown as the ratio of mRNA in human omental adipocytes, differentiated in vitro from the nonfat cells isolated from omental adipose tissue, to that in freshly isolated fat cells obtained by collagenase digestion of omental adipose tissue from female bariatric surgery patients. The ratios were derived from the Cp values and plotted on a log2 scale. Comparable amounts of total RNA were used for the mRNA analyses. The Cp values from which the ratios were determined for fat cells were calculated from the data shown in Table 2 and for in vitro differentiated adipocytes from Fain et al. [82, 83] or unpublished data. The red bars are for mRNAs whose expression in isolated fat cells was either the same or lower than in isolated nonfat cells.
