Regulatory Effect of Cinnamaldehyde on Monocyte/Macrophage-Mediated Inflammatory Responses

Figure 7

Effect of thiol compounds on CA inhibition of NO production and NF-κB-mediated luciferase activity. (a) RAW264.7 cells ($2\times {10}^6$ cells/ml) pretreated with L-cysteine for 30 min were incubated with indicated concentrations of CA in the presence of LPS (1 $\mu$g/ml) for 24 hours. NO levels in culture supernatant were determined by Griess assay. (b) TLR-4-expressing HEK293 cells cotransfected with the plasmid construct, NF-$\mu$B-Luc (1 $\mu$g/ml), and β-gal (as a transfection control) were treated with CA, L-cysteine or DTT in the presence or absence of TNF-$\mu$, PMA, or LPS for 18 h where luciferase activity was determined by luminometry. (c) CA and Suntide were incubated in kinase assay buffer for 30 min. The adduct was then identified by MALDITOF/MS analysis. ${}_{}{}^{**}P<.01$ and ${}_{}{}^{\#}P<.05$ represent significant difference compared to control or CA groups.

(a)

(b)

(c)