The p110𝛼 and p110𝛽 Isoforms of Class I Phosphatidylinositol 3-Kinase Are Involved in Toll-Like Receptor 5 Signaling in Epithelial Cells

Figure 7

Effects of p110 inhibitors on murine responses to flagellin in vivo. Female C57Bl/6 mice (6–12 weeks) were injected intraperitoneally with 200 $\mu$g of TGX-221 or PI-103 in 75% DMSO in PBS, or an equivalent volume of DMSO/PBS. Thirty min later, mice were injected intraperitoneally with 10 $\mu$g of E. coli H18 flagellin in 100 $\mu$l of PBS. Saphenous blood was taken after an addition 90 minutes and 3 hours. Sera were analyzed for IL-6 concentration by ELISA and results expressed as the ratio compared to flagellin plus DMSO control at each time point. Mean serum concentrations of IL-6 in the flagellin plus DMSO vehicle group were 1567 pg/ml at 90 minutes and 4915 pg/ml at 3 hours. $N=4$ per group. ${}^{*}P<.05$, TGX-221 versus DMSO, t-test.