The Traditional Japanese Formula Keishibukuryogan Inhibits the Production of Inflammatory Cytokines by Dermal Endothelial Cells
Figure 3
Effect of paeoniflorin COX-2 and iNOS protein expression. HDMEC cells were treated with 1 μg/mL LPS and/or 100 μg/mL of paeoniflorin for 24 h. COX-2 and iNOS protein expression in the cells was detected by a Western blot analysis, using β-actin as the internal control. The results of densitometric analysis, normalized with respect to β-actin using a bioimaging analyzer, are presented. Data are presented as the means ± S.D (n = 5). *P < .0001. (This experiment was repeated three times with similar results).