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Mediators of Inflammation
Volume 2010, Article ID 821940, 9 pages
http://dx.doi.org/10.1155/2010/821940
Research Article

Activation of CD147 with Cyclophilin A Induces the Expression of IFITM1 through ERK and PI3K in THP-1 Cells

1School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea
2Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea

Received 11 May 2010; Revised 29 June 2010; Accepted 30 June 2010

Academic Editor: Steven Kunkel

Copyright © 2010 Ju-Young Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

CD147, as a receptor for Cyclophilins, is a multifunctional transmembrane glycoprotein. In order to identify genes that are induced by activation of CD147, THP-1 cells were stimulated with Cyclophilin A and differentially expressed genes were detected using PCR-based analysis. Interferon-induced transmembrane 1 (IFITM1) was detected to be induced and it was confirmed by RT-PCR and Western blot analysis. CD147-induced expression of IFITM1 was blocked by inhibitors of ERK, PI3K, or NF- B, but not by inhibitors of p38, JNK, or PKC. IFITM1 appears to mediate inflammatory activation of THP-1 cells since cross-linking of IFITM1 with specific monoclonal antibody against it induced the expression of proinflammatory mediators such as IL-8 and MMP-9. These data indicate that IFITM1 is one of the pro-inflammatory mediators that are induced by signaling initiated by the activation of CD147 in macrophages and activation of ERK, PI3K, and NF- B is required for the expression of IFITM1.