Activation of CD147 with Cyclophilin A Induces the Expression of IFITM1 through ERK and PI3K in THP-1 Cells

Figure 6

IFITM1-mediated induction of MMP-9 expression requires ERK, PI3K, and NF-κB in THP-1 cells. (a) cells were preincubated with indicated concentrations of TPCK or JNK inhibitor or 10 $\mu$M of negative control for JNK inhibitor (J($-$)) for 30 min. Cells were then stimulated with 1 $\mu$g/mL of LPS or 10 $\mu$g/mL of anti-IFITM1 mAb for 24 hrs, and culture supernatants were collected for the measurement of MMP-9 activity using gelatin zymogram. (b) cells were preincubated with 10 $\mu$M of U0126 (U), SB203580 (SB), or LY294002 (LY) for 30 min. DMSO (D, 0.1%) was used as a vehicle control. Cells were then stimulated with 10 $\mu$g/mL of anti-IFITM1 mAb for 24 hr and MMP-9 activity was tested as in (a). These experiments were repeated twice with essentially the same results.

(a)

(b)