Distribution of Major Basic Protein on Human Airway following In Vitro Eosinophil Incubation
Figure 6
Immunolocalization of exogenously applied MBP in human airway. Results show representative images analyzing the fluorescence of immunolabeled MBP in human airway following incubation with MBP for 1 min and 1 h. Immunoreactive actin (green) and MBP (red) are shown. Brief Exposure. 1 min elicited limited MBP deposition to the epithelial and outer surfaces of the airway. However, longer 1 h exposure allowed some MBP to access cells within the media of the airway wall. The longer 1 h exposure was necessary for immunolocalization to be detected within the smooth muscle layer. This time-dependent effect was increased by greater concentrations of MBP (10-5 M) compared to lesser concentrations of MBP (10-7 M). In all control tissue no MBP content was detected (confocal microscopy 400x).
