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Mediators of Inflammation
Volume 2010, Article ID 858735, 9 pages
http://dx.doi.org/10.1155/2010/858735
Research Article

Apocynin Improves Insulin Resistance through Suppressing Inflammation in High-Fat Diet-Induced Obese Mice

1Department of Endocrinology, Nanjing Drum Tower Hospital, Nanjing University School of Medicine, 321 Zhongshan Road, Nanjing 210008, China
2Department of Medical Genetics, Nanjing University School of Medicine, 22 Hankou Road, Nanjing 210093, China
3Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, 22 Hankou Road, Nanjing 210093, China

Received 27 October 2010; Accepted 17 December 2010

Academic Editor: Chiara De Luca

Copyright © 2010 Ran Meng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We investigated the effects of apocynin on high-fat diet- (HFD-) induced insulin resistance in C57BL/6 mice. After 12 weeks of HFD, the mice that exhibited insulin resistance then received 5 weeks of apocynin (2.4 g/L, in water). Following apocynin treatment, fasting glucose, insulin, and glucose tolerance test showed significant improvement in insulin sensitivity in HFD-fed mice. We demonstrated that serum levels of tumor necrosis factor- (TNF- ), interleukin-6 (IL-6), and leptin were remarkably reduced with apocynin treatment. We also found that mRNA expression of TNF- , IL-6, and monocyte chemoattractant protein-1 (MCP-1) in the liver and mRNA expression of TNF- , IL-6, MCP-1, and leptin in adipose tissue were suppressed by apocynin. Furthermore, the activity of transcription factor NF- B in the liver was significantly suppressed with apocynin treatment. These results suggest that apocynin may reduce inflammatory factors in the blood, liver, and adipose tissue, resulting in amelioration of insulin resistance in HFD-fed mice.