Normal Human Gingival Epithelial Cells Sense C. parapsilosis by Toll-Like Receptors and Module Its Pathogenesis through Antimicrobial Peptides and Proinflammatory Cytokines
Quantification of mRNA expression levels of TLR-2, -4, -6, and -9 by gingival epithelial cells following infection with C. parapsilosis. Gingival epithelial cells were cultured with or without C. parapsilosis for 6 and 24 hours. Total RNA content was extracted from the cells following each culture period and was used for quantitative RT-PCR of the TLR-2, -4, -6, and -9 genes, as described in Section 2. Results are presented as a fold expression of the gene in the test sample compared to this gene expression in the control. Data are expressed as means ± SD from triplicate assays of three different experiments. (a) TLR-2 expression, (b) TLR-4 expression, (c) TLR-6 expression, and (d) TLR-9 expression. GEC: gingival epithelial cells.