Normal Human Gingival Epithelial Cells Sense C. parapsilosis by Toll-Like Receptors and Module Its Pathogenesis through Antimicrobial Peptides and Proinflammatory Cytokines
Quantification of mRNA expression levels of HBD-1, -2, -3, and -4 by gingival epithelial cells following infection with C. parapsilosis. Gingival epithelial cells were cultured with or without C. parapsilosis for 6 and 24 hours. Total RNA was extracted from the cells following each culture period and was used for quantitative RT-PCR of the HBD-1, -2, -3, and -4 genes, as described in Section 2. Results are presented as a fold expression of the gene in the test sample compared to this gene expression in the control. Data are expressed as means ± SD from triplicate assays of three different experiments. (a) HBD-1 expression, (b) HBD-2 expression, (c) HBD-3 expression, and (d) HBD-4 expression. GEC: gingival epithelial cells.