Research Article

Inflammatory Mediators Release in Urine from Mice Injected with Crotalus durissus terrificus Venom

Figure 2

Cytokine excreted in urine from BALB/c female mice. Urine of mice was obtained after i.p. injection with different amounts of Cdt as above described. The levels of IL-5 were determined after 15 min, for IL-4, IL-6, IL-10, and IFN-γ after 1 h. They were assayed by ELISA assay using monoclonal antibodies as the probe. TNF levels were determined after 1 h by standard assay with L929 cells. Each bar represents the mean value of samples from four experiments in different groups of five mice. Statistical differences between the treatments were ( ).
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