Mediators of Inflammation / 2011 / Article / Fig 8

Research Article

Dual Specificity Phosphatase 1 Regulates Human Inducible Nitric Oxide Synthase Expression by p38 MAP Kinase

Figure 8

iNOS protein expression in bone marrow macrophages from wild-type and DUSP1-deficient mice. Bone marrow-derived macrophages differentiated from wild-type (DUSP1+/+ and DUSP1-deficient (DUSP1−/−) mice were incubated with LPS (10 ng/mL) for 24 h, and iNOS protein was measured by Western blot. The gels are representatives of three separate experiments with similar results. Chemiluminescent signal was quantified, and iNOS expression was normalized to actin. Results are expressed as a percentage of LPS, mean ± S.E.M. ( ). One-way ANOVA with Bonferroni’s posttest was performed, and statistical significance is indicated with and .