Lipopolysaccharide Inhibits the Channel Activity of the P2X7 Receptor

Figure 4

LPS inhibits the ATP-induced EtBr uptake on P2X7R-HEK293 cells. (a) Representative images obtained from EtBr uptake protocols in nontreated cells (basal) after 30 min of treatment with 1 mM ATP alone (ATP) or plus 1 μg/mL LPS (ATP + LPS). (b) Summary of the time-dependent EtBr incorporation obtained with 1 mM ATP alone (open circles) or plus LPS (closed triangles); 𝑛=4. (c) LPS inhibition of ATP-induced EtBr uptake at different ATP concentrations (𝑃<0.05, unpaired t-test; 𝑛=3-4). Experiments were performed in the presence of 1% FBS.

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