Surface TLR2 and TLR4 Expression on Mature Rat Mast Cells Can Be Affected by Some Bacterial Components and Proinflammatory Cytokines

Figure 5

The effect of (a) LPS, (b) LAM, and (c) PGN on TLR4 expression on rat mast cells. Mast cells were incubated with bacterial components or cDMEM (control TLR4 expression on unstimulated cells referred to as 100%) for 1,  3,  6,  12, or 24 hours. LPS concentrations −1 ng/mL (dark bars) and 10² ng/mL (bright bars) and LAM and PGN concentrations −1 μg/mL (dark bars) and 10² μg/mL (bright bars). Results are presented as a percentage of control TLR4 expression. Each point represents the mean ± SEM of 6 independent experiments. **𝑃<.01. (d) Representative flow cytometry histogram showing TLR4 expression on rat mast cells treated with 1 ng/mL LPS for 3 h. (e) Representative flow cytometry histogram showing TLR4 expression on rat mast cells treated with 10² ng/mL LPS for 12 h. (f) Representative flow cytometry histogram showing TLR4 expression on rat mast cells treated with 10² ng/mL LPS for 24 h. (g) Representative flow cytometry histogram showing TLR4 expression on rat mast cells treated with 10² μg/mL LAM for 1 h. Filled histograms: untreated mast cells (control) and open histograms: LPS- or LAM-treated mast cells.