Antiasthmatic Effects of Hesperidin, a Potential Th2 Cytokine Antagonist, in a Mouse Model of Allergic Asthma

Figure 4

Dot plot pattern of intracellular cytokine staining. X-axis: PE intensity of GATA-3 and IL-17. Y-axis: FITC intensity of CD4. Isolated CD4+ T cells were cultured without (normal group) or with (stimulated group) anti-CD3, anti-CD28, rIL-6, TGF-beta, and with HPN (10, 1 μg/mL) added to RPMI medium and stained with FITC-conjugated mAb to CD4 and PE-conjugated mAb to GATA-3 or IL-17 (described in Section 2).