Lower Frequency of CD62L^high and Higher Frequency of TNFR2⁺ Tregs Are Associated with Inflammatory Conditions in Type 1 Diabetic Patients

Figure 1

Gating strategy used to analyze CD4⁺CD25^high regulatory T cells in peripheral blood. The whole blood was stained with antibodies against Treg cell surface molecules. Sample gated on lymphocytes according to forward and side scatter (FSC/SSC) (a). Plots showing gate for CD4⁺ T cells (b) and CD4⁺CD25^high cells (c). Cells in CD4⁺CD25^high gate gated on TNFR2⁺ (d) and CD62L^high cells (e).