Systemically Administered Ligands of Toll-Like Receptor 2, -4, and -9 Induce Distinct Inflammatory Responses in the Murine Lung

Table 1

TNF-α expression in the lung. Pulmonary TNF-α mRNA (RQ TNF-a/GAPDH cDNA) and TNF-α protein (pg/mg protein) expression 0, 2, 4, and 6 h after LTA-, LPS-, and CpG-ODN-stimulation. Significant differences to control values are indicated (𝑛=4; *𝑃<.05; bdl = below detection limit; M ± SEM; 𝑛=6/group).

	TNF-α mRNA (RQ TNF-α/GAPDH cDNA)	TNF-α protein (pg/mg protein)
0 h LTA	1.11 ± 0.11	0.00 ± 0.00 (bdl)
2 h LTA	5.79 ± 0.68*	16.05 ± 7.89*
4 h LTA	2.08 ± 0.36	0.00 ± 0.00 (bdl)
6 h LTA	1.72 ± 0.37	0.00 ± 0.00 (bdl)
0 h LPS	0.61 ± 0.07	0.00 ± 0.00 (bdl)
2 h LPS	14.31 ± 1.36*	24.36 ± 3.63*
4 h LPS	12.82 ± 2.05*	12.35 ± 2.74*
6 h LPS	12.70 ± 1.05*	9.78 ± 0.42*
0 h CpG	0.54 ± 0.02	0.00 ± 0.00 (bdl)
2 h CpG	19.88 ± 3.90*	12.81 ± 1.55*
4 h CpG	2.65 ± 0.67	2.70 ± 0.84
6 h CpG	2.74 ± 0.71	3.84 ± 2.92