IB inhibitory subunit expression in the cerebellum of autistic subjects. (a) Two independent western blot studies on cerebellar homogenates using IB antibody (dilution 1 : 1000). Lanes 1–4 and 9–11 represent the controls, and lanes 5–8 and 12–14 represent the autistic subjects. The upper panel shows actin bands (MW: 48 kd), and the lower panel shows the IB bands (MW: 35 kd). (b) Quantitative analysis after being normalized by actin. Data are shown as mean ± SE. White bars represent controls, and black bars represent autistic subjects. (c) Immunostaining of cerebellar sections using IB antibody (dilution 1 : 200). The expression of IB shown as very faint red color was detected in the neural cells (indicated by arrows) in autistic cerebellum (right panel), as well as in the matched controls (left panel). The image is viewed under high power microscopy. (d) Measurement of phospho-IB in the cerebellum of 7 autistic subjects and the age-matched controls with ELISA. .
