Emerging Role of PPAR-β/δ in Inflammatory Process Associated to Experimental Periodontitis

Figure 3

Effect of GW0742 on cytokines expression. At eight days after ligation, there was a substantial increase in TNF-α (a) and IL-1β (b) formation when compared with gingivomucosal tissue sections taken from the contralateral side (a, b). In contrast, GW0742 treatment significantly reduced TNF-α (a) and IL-1β (b) secretion. Immunohistochemical analysis of gingivomucosal tissues from ligated rats revealed positive staining for IL-1β (c). In gingivomucosal tissue of GW0742-treated rats no positive staining was observed for IL-1β (d). Densitometry analysis of immunocytochemistry photographs (𝑛=5 photos from each sample collected from all mice in each experimental group) for IL-1β was assessed (e). Myeloperoxidase activity (f), the total number of infiltrating leukocytes (g) and the Evans blue content (h) in gingivomucosal tissue were significantly increased by ligature compared to the contralateral side. GW0742 significantly reduced Myeloperoxidase activity (f), the total number of infiltrating leukocytes (g) and the Evans blue content (h). Data are means of mean ± S.E.M. from 𝑁=10 rats for each group. *𝑃<0.01 versus nonligated. °𝑃<0.01 versus ligated.