Emerging Role of PPAR-β/δ in Inflammatory Process Associated to Experimental Periodontitis
Figure 5
Effects of GW0742 on apoptosis. Immunohistochemistry for Bax and Fas-L showed positive staining in the gingivomucosal sections after ligature (a, e). The degree of positive staining for Bax and Fas-L was markedly reduced in GW0742-treated rats (b, f). Moreover, the degree of positive staining for Bcl-2 was markedly reduced in gingivomucosal sections obtained after ligature (c). The reduction of Bcl-2 expression was significantly attenuated by GW0742 treatment (d). In western blot analysis, basal level of Bax was present in the tissue from sham-operated rats (g, h). Bax band is more evident in the tissue from ligature-treated rats (g, h). The Bax band disappeared in the tissue from GW0742 treated (g, h). Moreover, a basal level of Bcl-2 was present in the tissue from sham-operated rats (i, j). The Bcl-2 band disappeared in the tissue from rats subjected to ligation (i, j). The Bcl-2 band is more evident in the tissue from ligated rats that received GW0742 (i, j). This figure is representative of at least 3 experiments performed on different experimental days. A representative blot of lysates obtained from 5 animals per group is shown, and densitometry analysis of all animals is reported. The results in (h, j) are expressed as mean ± S.E.M. from gingivomucosal tissues for each group. * versus nonligated group. ° versus ligated group.
