Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3: Downregulation of Inflammatory and Regulatory Genes
Figure 5
Effect of Plasmid with Gal-3 on Quantitative Expression of Cytokine Genes in lungs after Twelve Weeks of OVA Exposure. Relative mRNA levels of IL-10 (a) and TGF-β 1(b) gene expression in lungs from different groups of mice were determined by real-time quantitative PCR. Values were normalized with the rRNA gene used as an endogen. Black bars represent the OVA group (geometric mean ± SD, ), white bars the pEGFP group (geometric mean ± SD, ), shaded bars the pEGFP-Gal-3 group (geometric mean ± SD, ), and gray bars the SS group (geometric mean ± SD, ). The results show relative gene expressions, as determined by the ΔΔCT method, after twelve weeks of antigen exposure. Significant differences in IL-10 and TGF-β 1 (***) expression levels were obtained for the pEGFP-Gal-3 versus the OVA and pEGFP groups. In the case of empty plasmid, no significant differences were found with respect to the OVA group.
