Progesterone Administration Modulates Cortical TLR4/NF-κB Signaling Pathway after Subarachnoid Hemorrhage in Male Rats
Figure 2
(a) Representative autoradiogram of TLR4, NF-κB, ICAM-1, and MCP-1 in the cortex. We observed TLR4 at 89 kDa, NF-κB at 65 kDa, ICAM-1 at 59 kDa, MCP-1 at 14 kDa, and a loading control GAPDH at 37 kDa. Lane 1, control group; Lane 2, SAH groups; Lane 3, SAH + vehicle group; Lane 4, SAH + progesterone group. It shows that the expression of TLR4/NF-κB related proteins increased after SAH and inhibited by progesterone administration. (b) Quantitative analysis of the Western blot results for TLR4, NF-κB, ICAM-1, and MCP-1. It shows that the levels of TLR4, NF-κB, ICAM-1, and MCP-1 in SAH groups are significantly higher than that in control. After progesterone administration, the TLR4/NF-κB related agents were significantly down-regulated as compared with SAH + vehicle group. Bars represent the mean ± SD (, each group). * compared with control group, ** compared with control group, # compared with SAH + vehicle group, ## compared with SAH + vehicle group, ns compared with SAH group.
